Figure 1. 100 μM H₂O₂ induced primary cultured SD rat retinal cell apoptosis, which was associated with an increase in [Ca²⁺]_i at the early stage of apoptosis.

A, B: Quantitative data of cell viability and [Ca²⁺]_i under different concentrations of H₂O₂ treatments for 2 hrs; D, E: Cell viability and [Ca²⁺]_i quantitative data at different time points after 100 μM H₂O₂-induced stress; C, F: The overlay figure of the representative statistical significance for B and E; G: Apoptosis assay using Hoechst 33342 staining at different time points after 100 μM H₂O₂-induced stress; H: Quantitative data of G. Values shown are the Mean ±SD. *represents P<0.05, **represents P<0.01 and ***represents P<0.001 compared with the control group by one-way ANOVA statistical analysis. (A, D, H: n indicates 3 independent replicates with 4 samples per condition per experiment; B, E: n indicates 3 independent replicates with 5 samples per condition per experiment.).