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. 2013 Nov 5;8(11):e77218. doi: 10.1371/journal.pone.0077218

Figure 3. Sources of increased [Ca2+]i induced by 100 μM H2O2 treatment for 2 hrs and 10 μM βE2 treatment for 0.5 hrs.

Figure 3

A, B: The effects of different concentrations of EGTA treatment for 24 hrs on cell viability and EGTA treatment for 1 hr on [Ca2+]i; C: The overlay figure for B; D-F and G-I: The effect of different concentrations of EGTA pretreatment for 1 hr before H2O2 or βE2 application on the alteration of cell viability and [Ca2+]i induced by H2O2 (D-F) or βE2 (G-I); F and I: The representative overlay figure for E and H. Values shown are the Mean ±SD. *represents P<0.05, **represents P<0.01 and ***represents P<0.001 compared with the control group; # represents P<0.05, ## represents P<0.01 and ### represents P<0.001 compared with the H2O2 or βE2 application groups by one-way ANOVA statistical analysis. (A, D, E: n indicates 4 independent replicates with 5 samples per condition per experiment; B, G, H: n indicates 4 independent replicates with 6 samples per condition per experiment.).