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. 2013 Nov 6;33(45):17863–17873. doi: 10.1523/JNEUROSCI.2196-13.2013

Figure 1.

Figure 1.

Synaptic terminal overgrowth at the NMJs in the shot mutant. A, Sample confocal images of muscle 4 type Ib NMJs of WT, shotvv/shotvv (shotvv), shotvv/shot3 and UAS-shot-FL-GFP/+ shotvv/shot3; elav-Gal4/+ (shotvv/shot3 + rescue) stained for the postsynaptic DLG (red), presynaptic DVGLUT (green), and the membrane marker HRP (blue). Scale bar, 50 μm. B, Sample confocal images of muscle 4 type Ib NMJs of UAS-dcr2, elav-Gal4/UAS-shot-RNAi (Neuronal shot RNAi), and UAS-dcr2; G7-Gal4/+; UAS-shot-RNAi/+ (Muscle shot RNAi). Muscle 4 type Ib bouton number of the genotypes listed in A are quantified in C. **p < 0.001, compared with WT. *p < 0.05, compared with WT. Phenotypes listed in B with addition of UAS-dcr2;; elav-Gal4/+ (control) were quantified in D. **p < 0.001, compared with control. n > 15 for each genotype. Error bars indicate SEM.