Figure 2.

Rescue constructs missing the Rod domain and Plakin domain rescue the NMJ overgrowth phenotype of shot. A, Schematic representation of Short Stop and the deletion constructs. Shot ΔCalponin is a Shot C isoform, which lacks one of the two Calponin homology domains. The rest of the constructs were derived from Shot A isoform represented in A as Shot FL (full-length). All constructs are COOH GFP-tagged. B, Sample confocal images of two nerves in the larvae expressing deletion constructs with the strong neuronal driver BG380-Gal4 in the WT background. The nerves are stained for GFP (green) to label the expression of the constructs and HRP (red) to label the neuronal membrane. Scale bar, 50 μm. C, Quantification of the muscle 4 type Ib bouton number in elav-Gal4/+ (Control), UAS-shot-FL/+; elav-Gal4/+ (shot FL o/e), shot^vv/shot³; elav-Gal4/+ (shot), UAS-shot-FL; shot^vv/shot^3; elav-Gal4/+ (shot + Shot FL), shot^vv/shot³, UAS-shot-ΔCalponin; elav-Gal4/+ (shot + ΔCalponin), UAS-shot-ΔGas2/+; shot^vv/shot³; elav-Gal4/+ (shot + ΔGas2), shot^vv/shot³, UAS-shot-ΔEFHand; elav-Gal4/+ (shot + ΔEF); shot^vv/shot³, UAS-shot-ΔRod1; elav-Gal4/+ (shot + ΔRod), shot^vv/shot³, UAS-shot-ΔPlakin; elav-Gal4/+ (shot + ΔPlakin). **p < 0.001, compared with shot^vv/shot³; elav-Gal4/+ (shot). n > 15 for each genotype. Error bars indicate SEM.