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. 2013 Nov 6;3:72. doi: 10.3389/fcimb.2013.00072

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Copyright © 2013 Wang, Xiang, Feng, Srinivas, Zhang, Lin and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein purification and identification. (A) Protein purification. The purified protein was determined by SDS-PAGE. M: Molecular weight of marker proteins; Lane 1–4: the purified proteins for pACYC-Duet-scFv, pACYC-Duet-scFv-skp, pET28a-scFv, and pET32a-scFv, respectively. (B) Western blotting results. Lane 1–4: the detected bands for pACYC-Duet-scFv, pACYC-Duet-scFv-skp, pET28a-scFv, and pET32a-scFv, respectively. (C) ELISA. TLH antigen were coated on 96-well plates in triplicate (5 μ g/mL, 100 μ L/well), and the purified scFv proteins were added to the reaction wells after blocking and washing. The binding activities of the four purified proteins were determined using an anti-6×His tag antibody. (D) Quantitative result of ELISA^*, P < 0.05, compared with control antibody treatment.