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. Author manuscript; available in PMC: 2013 Nov 6.
Published in final edited form as: Nature. 2008 Sep 21;455(7214):10.1038/nature07312. doi: 10.1038/nature07312

Figure 1. Sae2 and Exo1 function at different steps in DSB resection.

Figure 1

a. Map of the chromosome XV region containing the ade2 direct repeat. The ade2-I allele is cleaved by I-SceI to yield the 2.3-kb and 1.7-kb cut fragments that disappear over time concomitant with repair to the 5-kb SSA product. b. SSA efficiency as indicated by sectoring on YPD plates and survival on YPRaf/Gal plates. Ade+ (white) colonies arise from ade2 (red) colonies by SSA during growth on YPD due to leaky expression of I-SceI. c. SSA physical assay: Monitoring of resection and repair of the I-SceI induced DSB by southern blot analysis of NheI/EagI digested genomic DNA. d. Quantitation of the ADE2 hybridization signal on native versus denatured DNA samples bound on nylon membrane. The means from multiple experiments from three independent inductions are presented, error bars indicate s.d.. * Denotes statistical significance of P<0.02 (unpaired t-test).