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. Author manuscript; available in PMC: 2013 Nov 6.
Published in final edited form as: Nature. 2008 Sep 21;455(7214):10.1038/nature07312. doi: 10.1038/nature07312

Figure 2. Sgs1 and Exo1 function redundantly in DSB resection.

Figure 2

a. SSA plating efficiency as described in Fig. 1b. b. SSA physical assay as described in Fig. 1c. c. Schematic representation of MAT switching: the 0.9-kb MATa StyI fragment is cleaved by HO resulting in a 0.7-kb cut fragment that disappears over time concomitant with repair to an 1.8-kb MATα fragment. d. MAT switching assay: HO cut fragment processing and gene conversion efficiency as monitored by southern blot analysis of StyI digested genomic DNA. e. MAT switching assay on sgs1Δ exo1Δ cultures complemented with mutant or wild-type SGS1.