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. Author manuscript; available in PMC: 2014 Oct 10.
Published in final edited form as: Mol Cell. 2013 Oct 10;52(1):10.1016/j.molcel.2013.09.007. doi: 10.1016/j.molcel.2013.09.007

Figure 3. Joint molecules accumulate in mph1Δ, mus81Δ and sgs1Δ mutants.

Figure 3

A. Schematic showing the expected sizes of parental fragments and joint molecules after digestion with ApaLI and PvuII, and their expected migration by neutral 2D gel electrophoresis. B. 2D gel analysis of DNA isolated from the indicated strains 1.5, 2.5 or 4.5 h after HO induction. The orange and green triangles correspond to inter-sister and ectopic JM, respectively; * corresponds to HO collapsed inter-sister JMs. C. An IS-JM forms by invasion of an uncut sister chromatid by the HO cut sister. If the IS-JM is not fully gap filled and ligated then HO would cleave only the newly synthesized strand and its complement and not the D-loop. Branch migration of the junction towards the DSB would release the junction resulting in Y-shaped molecules of 11.5 or 9.5 kb. Ch-specific probes were used to verify formation of inter-sister JMs.