Table 4.
Identification of differentially expressed metabolites in the plasma that may account for the discrimination between normal and model rats.
| No. | Metabolites | Formula | Obsd. [M + H]+/[M − H]− (m/z) | Content variancec | FCd | P e value |
|---|---|---|---|---|---|---|
| 1 | LysoPC (22:5 (7Z, 10Z, 13Z, 16Z, 19Z)) | C30H52NO7P | 568.3436a | ↓ | −2.33 | 0.018 |
| 2 | 5-Dehydro-4-deoxy-D-glucarate | C6H8O7 | 191.0197a | ↑ | 2.56 | 0.022 |
| 3 | LysoPC (17:0) | C25H52NO7P | 508.3403a | ↓ | −1.42 | 0.030 |
| 4 | 5α-Tetrahydrocorticosterone | C21H34O4 | 349.2378a | ↑ | 2.56 | 0.035 |
| 5 | PC (13:0/0:0) | C21H44NO7P | 452.2774a | ↑ | 1.25 | 0.021 |
| 6 | 17-phenyl trinor PGF2α methyl ester | C24H34O5 | 404.2438b | ↑ | 2.01 | 0.017 |
| 7 | PC (0:0/18:0) | C26H54NO7P | 524.3720b | ↓ | −1.35 | 0.042 |
| 8 | LysoPC (18:2 (9Z, 12Z)) | C26H50NO7P | 520.3424b | ↓ | −1.56 | 0.030 |
| 9 | LysoPC (16:0) | C24H50NO7P | 496.3450b | ↓ | −1.78 | 0.016 |
| 10 | LysoPC (22:6 (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)) | C30H50NO7P | 568.3421b | ↓ | −2.60 | 0.026 |
aObserved at ES− mode [M − H]−; bobserved at ES+ mode [M + H]+ .
c↑: content increased; ↓: content decreased.
dFold change was calculated as the ratio of the mean metabolite levels between two groups. A positive value of fold change indicates a relatively higher concentration of metabolites, while a negative value of fold change indicates a relatively lower concentration of metabolites in model rats as compared to normal rats.
e P values were calculated from two-tailed Mann-Whitney U test with a threshold of 0.05.