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. 2011 Feb 22;4(2):252–262. doi: 10.1111/j.1751-7915.2010.00234.x

Figure 4.

Figure 4

A. Scheme of the gene inactivation of epzP. aac(3)IV, apramycin resistance gene; Paac, promoter of the apramycin resistance gene; FRT, FLP recognition target; oriT, origin of transfer from RK2; neo, kanamycin resistance gene. Out of scale.
B. Confirmation of the genotype of single‐cross‐over mutants (SCO) and double‐cross‐over mutants (DCO). Lane M: marker, C1: cosmid 8‐4D (wild type), C2: cosmid epzKG03 (ΔepzP). Lanes 4, 6 and 7: SCO mutants. Lanes 1, 2 and 5: DCO mutants.
C. HPLC/MS analysis of the formation of endophenazine A, endophenazine B and phenazine‐1‐carboxylic acid (PCA) in S. cinnamonensis DSM 1042 (wild type), the ΔepzP mutant and the ΔepzP mutant complemented with intact epzP.