Table 3.
N. fowleri detection in seeded 1 L lake water samples.
| Seed level | Direct PCR without culture | Culture followed by PCR confirmation | ||||||
|---|---|---|---|---|---|---|---|---|
| IMS | No IMS | IMS | No IMS | |||||
| CT | Pos† no. | CT | # Pos | CT | Pos no. | CT | # Pos | |
| 14 | 39.1 | 1 | 38.0 | 1 | Negative | 0 | 38.9 | 1 |
| 63 | 38.5 | 3 | 40.5 | 2 | 32.8 | 1 | 34.6 | 2 |
| 388 | 35.1 | 4 | 36.7 | 4 | 27.8 | 4 | 38.3 | 1 |
| 4,900 | 31.9 | 4 | 33.2 | 4 | 28.9 | 4 | 35.7 | 4 |
| 11,300 | 29.83 | 4 | 29.96 | 4 | 23.99 | 4 | 34.87 | 4 |
| 129,000 | 27.87 | 4 | 28.19 | 4 | 25.38 | 4 | 31.57 | 4 |
†Number of positive results out of four replicate tests (all conditions were repeated four times).