Table 1.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Relevant features | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli strains | ||
| E. coli EC101 | Cloning host, repA+ kmr | Law et al. (1995) |
| E. coli EC101‐pNZ‐M.BbrII + M.BbrIII | EC101 harbouring pNZ8048 derivative containing bbrIIM and bbrIIIM. | O'Connell Motherway et al. (2009) |
| Lactococcus lactis strains | ||
| L. lactis NZ9000 | MG1363, pepN::nisRK, nisin‐inducible overexpression host | de Ruyter et al. (1996) |
| L. lactis NZ9000‐ pNZ‐galA | NZ9000 containing pNZ‐galA | This study |
| L. lactis NZ9000‐ pNZ‐galAT | NZ9000 containing pNZ‐galAT | This study |
| L. lactis NZ9000‐ pNZ‐galG | NZ9000 containing pNZ‐galG | This study |
| L. lactis NZ9000‐ pNZ‐galR | NZ9000 containing pNZ‐galR | This study |
| Bifidobacterium sp. strains | ||
| B. breve UCC 2003 | Isolate from nursling stool | Mazéet al. (2007) |
| B. breve UCC2003‐galA‐967 | pORI19‐tet‐galA‐967 insertion mutant of UCC2003 | This study |
| B. breve UCC2003‐galG‐410 | pORI19‐tet‐galG‐410 insertion mutant of UCC2003 | This study |
| B. breve UCC2003‐galC‐701 | pORI19‐tet‐galC‐701 insertion mutant of UCC2003 | This study |
| B. breve UCC 2004 | Isolate from human faeces | UCC |
| B. breve UCC 2005 | Isolate from human faeces | UCC |
| B. breve UCC 2007 | Isolate from human faeces | UCC |
| B. breve JCM 7017 | Isolate from human faeces | JCM |
| B. breve JCM 7019 | Isolate from infant faeces | JCM |
| B. breve NCFB 2257 | Isolate from infant intestine | NCFB |
| B. breve NCFB 2258 | Isolate from infant intestine | NCFB |
| B. breve NCTC 11815 | Isolate from infant intestine | NCTC |
| B. breve CCUG 43878 | Isolate from human faeces | CCUG |
| B. adolescentis CIP 64.61 | Isolate from human intestine | CIP |
| B. adolescentis DSM 20083 | Isolate from human intestine | DSM |
| B. adolescentis NCFB 2229 | Isolate from human intestine | NCFB |
| B. adolescentis NCFB 2204 | Isolate from human intestine | NCFB |
| B. adolescentis LMG 10502 | Isolate from human intestine | LMG |
| B. animalis JCM 20097 | Isolate from calf faeces | JCM |
| B. animalis DSM 20105 | Isolate from chicken faeces | DSM |
| B. bifidum NCIMB 8810 | Isolate from human intestine | NCIMB |
| B. bifidum LMG 11041 | Isolate from animal intestine | LMG |
| B. dentium Bd1 | Isolate from human dental caries | Ventura et al. (2009b) |
| B. dentium ATCC 27678 | Isolate from human dental caries | ATCC |
| B. longum JCM 7050 | Isolate from human faeces | JCM |
| B. longum JCM 7052 | Isolate from human faeces | JCM |
| B. longum JCM 7053 | Isolate from infant faeces | JCM |
| B. longum JCM 7056 | Isolate from infant faeces | JCM |
| B. longum CIP 64.63 | Isolate from infant intestine | CIP |
| B. longum CCUG 30698 | Isolate from human abdomen | CCUG |
| B. longum NCIMB 8809 | Isolate from human faeces | NCIMB |
| B. longum CCUG 15137 | Isolate from human | CCUG |
| B. longum/infantis CCUG 18157 | Isolate from human faeces | CCUG |
| B. longum subsp. infantis CCUG 52486 | Isolate from human faeces | CCUG |
| B. longum subsp. infantis ATCC 15697 | Isolate from human faeces | ATCC |
| B. infantis NCDO 2205 | Isolate from infant intestine | NCDO |
| B. pseudocatenulatum LMG 10505 | Isolate from infant faeces | LMG |
| B. pseudocatenulatum NCIMB 8811 | Isolate from infant faeces | NCIMB |
| B. pseudolongum NCIMB 2244 | Isolate from swine faeces | NCIMB |
| B. pseudolongum DSM 20095 | Isolate from chicken faeces | DSM |
| B. glodosum JCM 5820 | Isolate from animal rumen | JCM |
| B. glodosum JCM 7092 | Isolate from bovine rumen | JCM |
| B. thermophilum JCM 7027 | Isolate from swine faeces | JCM |
| Plasmids | ||
| pNZ8150 | Cmr, nisin‐inducible translational fusion vector | Mierau and Kleerebezem (2005) |
| pNZ‐galA | Cmr, pNZ8150 derivative containing translational fusion of gala‐encoding DNA fragment without signal sequence to nisin‐inducible promoter | This study |
| pNZ‐galAT | Cmr, pNZ8150 derivative containing translational fusion of truncated galA‐encoding DNA fragment (from bases 90 to 1330)to nisin‐inducible promoter | This study |
| pNZ‐galG | Cmr, pNZ8150 derivative containing translational fusion of galG‐encoding DNA fragment without signal sequence to nisin‐inducible promoter | This study |
| pNZ‐galR | Cmr, pNZ8150 derivative containing translational fusion of galR‐encoding DNA fragment without signal sequence to nisin‐inducible promoter | This study |
| pNZ44 | pNZ8048 containing constitutive p44 promoter from Lactococcal chromosome | McGrath et al. (2001) |
| pSKEM | E. coli bifidobacterial shuttle vectoe harbouring pCIBA089 rep | Cronin et al. (2007) |
| pCIB‐p44 | Complementation vector; pNZ44 where repA has been replaced with pCIBA089 rep | This study |
| pCIB‐p44‐galG | pCIB‐p44 derivative with galG transcriptionally fused to p44 promoter | This study |
| pAM5 | pBC1‐puC19‐Tcr | Alvarez‐Martín et al. (2007) |
| pORI19 | Emr, repA‐, ori+, cloning vector | Law et al. (1995) |
| pORI19‐tet‐galA | Internal 967 bp fragment of galA and tetW cloned in pORI19 | This study |
| pORI19‐tet‐galG | Internal 410 bp fragment of galG and tetW cloned in pORI19 | This study |
| pORI19‐tet‐galC | Internal 701 bp fragment of galA and tetW cloned in pORI19 | This study |
ATCC, American type culture collection; CCUG, Culture Collection of the University of Goteborg; CIP, Collection de l'Institut Pasteur; DSM, German Collection of Microorganisms and Cell Cultures; JCM, Japan Collection of Microorganisms; LMG, Belgian Co‐ordinated Collection of Microorganisms; NCDO, National Collection of Dairy Organisms; NCFB, National Collection of Food Bacteria; NCIMB, National Collection of Industrial and Marine Bacteria; NCTC, National Collection of Type Cultures; UCC, University College Cork Culture Collection.