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. 2011 Aug 17;4(5):651–662. doi: 10.1111/j.1751-7915.2011.00263.x

Figure 4.

Figure 4

Effect of CBD500 duplication on binding properties and lytic activity. A. Schematic representation of tandem CBD500 fusion proteins and the single CBD500 reference proteins. GFP = green fluorescent protein; EAD500 = enzymatically active domain (l‐alanoyl‐d‐glutamate peptidase) of Listeria phage endolysin Ply500; CBD500 = cell wall‐binding domain of Ply500. B. Overlay of SPR sensorgrams showing association and dissociation phases of GFP_CBD500 and GFP_CBD500‐500, respectively, to the cell walls of L. monocytogenes WSLC 1042, measured at a protein concentration of 50 nM. RU = relative response units. C. Relative lytic activity of Ply500 (○) and EAD_CBD500‐500 (Inline graphic) on WSLC 1042 cells at different NaCl concentrations. All assays were carried out in triplicate.