Figure 1:

HPLC chromatograms of pure standards (A) and C. roseus crude aqueous extract alkaloids (B). Stationary phase—reverse-phase Microsorb-MV C₁₈ column (150 x 4.6 mm); mobile phase-methanol, phosphate buffer, acetonitrile; flow-rate of mobile phase-0.2 ml/min; detection wavelength-254 nm; injection sample volume-20 μl.