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. 2013 Aug 29;1(3):e00074. doi: 10.1002/phy2.74

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© 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Efficacy of the morpholino antisense oligonucleotide targeting the splice acceptor site of podocalyxin exon 2 (podxlMOex2). (A) podxlMOex2 caused missplicing of the mRNA as detected by the presence of the altered PCR product, which is ∼360 bp smaller (arrow) than the wild-type product (arrowhead). The altered PCR product was present from 1 to 4 dpf. (B and C) Sequencing of the altered product revealed an in-frame deletion of lower 363 bp (73%) from exon 2, resulting in the deletion of 53% of the mucin domain from Podocalyxin in the podxlMOex2 morphants.