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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Biochem Pharmacol. 2013 Aug 22;86(9):1263–1271. doi: 10.1016/j.bcp.2013.08.019

Figure 6. Competition of peptides with CXCL12 for binding to CXCR7.

Figure 6

MDA-MB-231 cells stably transduced with CXCR7 were incubated on ice with 300 ng/ml CXCL12, 300 μM peptide, or vehicle control prior to adding ≈ 10 ng/ml CXCL12 fused to Gaussia luciferase (CXCL12-GL). 231-control cells without CXCR7 also were incubated with vehicle control before adding CXCL12-GL to account for CXCR7-independent binding to cells. Cells remained on ice for 45 minutes and then were washed with PBS before quantifying cell-associated CXCL12-GL. Graph shows mean values + SEM for photon flux (n = 4 per condition). **, p < 0.01 and *, p < 0.05 relative to 231-control cells.