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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Biochem Pharmacol. 2013 Aug 22;86(9):1263–1271. doi: 10.1016/j.bcp.2013.08.019

Figure 7. Effects of peptides on CXCR7-dependent scavenging of CXCL12.

Figure 7

231-CXCR7 cells were incubated with 300 ng/ml CXCL12, 300 μM peptide, or vehicle control along with ≈ 10 ng/ml CXCL12-GL for one hour at 37°C. 231-control cells incubated with vehicle control and CXCL12-GL were used to measure CXCR7-independent uptake of CXCL12-GL. Cells were acid-washed to removed extracellular chemokine prior to quantifying internalized CXCL12-GL bioluminescence. Graph shows mean values + SEM for bioluminescence quantified as photon flux (n = 4 per condition). ***, p < 0.005; **, p < 0.01; *, p < 0.05 relative to uptake in 231-CXCR7 cells incubated with vehicle control.