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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Wiley Interdiscip Rev Dev Biol. 2013 Feb 4;2(6):10.1002/wdev.107. doi: 10.1002/wdev.107

Figure 3. Comparing image quality of SPIM and laser scanning multi photon microscopy.

Figure 3

Multiphoton optical sections are shown for stage-16 Drosophila embryos stained to label nuclei. A) The top two images are of a live embryo expressing GFP-histone. The images were taken using the two-photon SPIM technique of "simultaneous multiview imaging" (SiMView) and were kindly provided by Philipp J. Keller48. B) The bottom two images are of a fixed emrbyo stained with SYTOX Green and were acquired using standard two-photon laser scanning microscopy. The embryo's dorsal / ventral direction is shown from top to bottom in each image. Optical sections were selected through the midplane of each three dimensional embryo image to show, from left to right, its anterior / posterior (left) and it's left / right (right) directions.