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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Vascul Pharmacol. 2013 Jun 6;59(0):10.1016/j.vph.2013.06.001. doi: 10.1016/j.vph.2013.06.001

Fig. 4.

Fig. 4

IL-6 repression by progesterone stimulation.(A) Protein expression (pg/µL) of IL-6 determined by cytokine array. HUVECs were treated with LPS (solid bars) or in combination with progesterone (hatched bars) for the indicated times. Graphs depict an average of three biological replicates. *p < 0.01, **p < 0.001. (B) qPCR confirmation of IL-6 expression. HUVECs were treated with LPS (solid bars) or in combination with progesterone (hatched bars) for the indicated times. Ct values were normalized to GAPDH and made relative to PR infected HUVECs in the absence of both LPS and progesterone (red hatched bar). GFP infected cells (first three bars) were used as a control and did not respond to progesterone. Graphs depict an average of three biological replicates run in triplicate. *p < 0.01, **p < 0.001, ***p < 0.0001, and ****p < 0.00001. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)