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. Author manuscript; available in PMC: 2013 Nov 6.
Published in final edited form as: J Microbiol. 2012 Apr 27;50(2):10.1007/s12275-012-1349-5. doi: 10.1007/s12275-012-1349-5

Fig. 3.

Fig. 3

Examination of cells and EPS in cell pellets or clumps derived from different M. xanthus strains. Panels A and B: pellets of 1 d cultures of SW504 (ΔdifA, and SW810-ΔepsA was similar in appearance) and DK10410 (ΔpilA) collected by centrifugation at 13,000 ×g for 5 min, respectively; panels C and D: clumps taken directly from 1 d cultures of DK1622 (Wt) and DK3088 (stk), respectively; panels E and F: clumps from 4 d cultures of DK1622 and DK3088, respectively; panels G and H: clumps from 12 d cultures of DK3088. Samples were counterstained with 5 μM SYTO 9, 5 mM CTC and 5 μg/ml Alexa 633-WGA in MOPS buffer. The panels are the overlay images of SYTO 9 (green) and Alexa 633-WGA (blue) signals, the upper small frames in the panels are the overlay images of CTC (red) and Alexa 633-WGA (blue) signals, and the lower frames are Alexa 633-WGA (blue) images, except that the upper small frame in the panel H shows the CTC (red) signal only. Bars represent 20 μm.