Figure 2. Apoptosis in renal tubular LLC-PK1 cells during in vitro I/R.

Cells were treated with antimycin A (12.5 to 100 μM) and 5 mM 2-deoxyglucose for 1.5 h to induce ischemia (I) injury followed by reperfusion (R) with growth medium for 24 h. Cell apoptosis was performed by Annexin V and PI dual staining and determined by flow cytometry. Data are presented as the means ± SDs in three independent experiments. **P < 0.01 as compared with vehicle control group. Ann V: annexin v.