Figure 5. Mefloquine treatment does not affect NAD⁺ biosynthesis.

mRNA expression levels of 9 genes involved in NAD⁺ biosynthesis were measured in cultured cochlea without mefloquine treatment, or treated with 50 µM mefloquine by quantitative RT-PCR. *Significantly different from control (a) (P < 0.05). NAD⁺ levels (b) and NAD⁺/NADH ratios (c) were measured in the homogenates of the cultured cochleae without mefloquine treatment (untreated), or treated with 35 µM mefloquine alone. 35 Mef = 35 µM mefloquine, and 20 NAD⁺ = 20 mM NAD⁺.