Figure 6. Mefloquine increases TUNEL-positive spiral ganglion neurons, while NAD⁺ prevents the increases.

Green fluorescence indicates nuclear DNA fragmentation in the spiral ganglion neurons in cultured cochlea without mefloquine treatment (a), or treated with 35 µM mefloquine alone (b) or 35 µM mefloquine + 20 mM NAD⁺ (c). TUNEL-positive cells were quantified in the spiral ganglion neuron regions (d). *Significantly different from control (P < 0.05). **Significantly different from mefloquine treatment alone (P < 0.05). 35 Mef = 35 µM mefloquine, and 20 NAD⁺ = 20 mM NAD⁺. Arrows indicate TUNEL-positive cells.