Table 1. Primer sequences and annealing temperature for qRT-PCR.

Gene	Forward primer	Reverse primer	Annealing temperature (°C)
NAPRT	AGGACTGTATGCGCTTTCTTC	CCAGAGCAATCAAGGGCTCG	60
NMPRT	GCAGAAGCCGAGTTCAACATC	TTTTCACGGCATTCAAAGTAGGA	60
QNS	CTCCTGCATTCGCTCCAAGTT	CCGGTGCATTATAGGCATCCC	60
NmNAT1	TTCAAGGCCTGACAACATCGC	GAGCACCTTCACAGTCTCCACC	53
NmNAT2	ATGACCGAGACCACAAAGACC	ACAATCCCGCCAATCACAATAA	53
NmNAT3	ATCACGAATATGCACCTGCG	ATTGACGGGTGAGATGATGCC	63
Nrk1	TCATTGGAATTGGTGGTGTGAC	CAACAGGAAACTGCTGACATCAT	63 (for 1 min)
Nrk2	CTCGGACACCCACGTACTC	CACGGTCAGGAAGTAGCGTT	62
QPRT	CTGGACAACCTCACCCAGTT	GTCCATTCTGGTGGCATCTT	63