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. 2013 Nov 6;8(11):e79863. doi: 10.1371/journal.pone.0079863

Figure 5. ON 01910.Na induced more cell death, mitotic cell arrest and hyperphosphorylation of RanGAP1 in DLBCL cell lines (HT and SU-DHL-5) than in non-neoplastic LCL.

Figure 5

(A) MTT assay shows the ID50 of ON 01910.Na is around 0.031 μM for DLBCL lines. (B) ON 01910.Na shows relatively selective cytotoxicity to DLBCL by flow cytometry analysis. (C) ON 01910.Na induces more evident mitotic cell arrest (G2/M, right panel) for DLBCL lines at the concentration between 0.016 and 0.032 μM on cell cycle analysis with flow cytometry. (D) Along with cell death, immunoblotting shows ON 01910.Na induces hyperphosphorylation of RanGAP1, increased expression of RanGAP1.SUMO1 but decreased expression of free unmodified RanGAP1. No viable SU-DHL-5 cells were available for immunoblotting at 0.5 μM of ON 01910.Na.