Table 3. Average number of synonymous substitutions per synonymous site (dS) and non-synonymous substitutions per non-synonymous site (dN) at each domain and individual codons affected by positive selection.
|
|
Positive selected sites
|
||||||
|---|---|---|---|---|---|---|---|
| Domain | dN (SE) | dS (SE) | FEL |
REL
|
MEME | FUBAR | |
| D0a | 0.103 (0.023) | 0.113 (0.035) | 39, 40, 52 | 39, 40, 52 | 32, 40, 52 | 25, 40 | |
| D0b | 0.005 (0.004) | 0.016 (0.012) | - | - | - | - | |
| D1 | 0.081 (0.013) | 0.102 (0.024) | 244, 262. 266, 281 | 244, 262. 266, | 229, 258, 262, 266, 277 | 254, 260, 262, 266 | |
| D2 | 0.050 (0.009) | 0.066 (0.018) | 314, 328, 330, 341 | 314, 328, 330, 341 | 314, 328, 330, 341, 345, 397 | 314, 328, 330 | |
| STC-A | 0.047 (0.018) | 0.158 (0.069) | 429, 438, 439, 455 | 455 | 455 | 429 | |
| STC-I | 0.033 (0.008) | 0.069 (0.020)* | |||||
No statistical differences were found between dN and dS substitution in Ig-like domains and STC-A domain or by SLAC method. Codon positions are according to the alignment shown in Figure 1. The C-terminal region including stem, transmembrane and cytoplasmatic domains (STC) was split according to their function: STC-A = activator tail and STC-I = inhibitor tail. (SE): Standard error. * p < 0.04.