Figure 2. β-glu6 (G) modulates the PI3K/Akt signaling pathway.

Macrophages were incubated with β-glu6 (100 μg/mL) alone for 2 h or pre-treated with anti-TLR2, anti-TLR4 or anti-Dectin-1 antibodies for 1 h. The Akt (Thr308), PTEN, PDK-1, and GSK-3β phosphorylation were detected by western blot assay, and beta-actin was used as a loading control (A). The band intensities were analyzed by Quantity One software (B, C, D and E). The numbers above the bars stand for the G/PBS ratio. Data shown represent one of three independent experiments.