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. 2013 Nov 6;8(11):e80501. doi: 10.1371/journal.pone.0080501

Table 2. Kinetic analysis of purified rhPGRN binding to amine-coupled mAbs, as determined via SPR.

mAb buffer ka (1/Ms)a x 106 kd (1/s)a x 10-4 KD (pM)
2B2 PBST 7.4 6.13 3.35 55
2B2 TBST 7.4 7.69 3.35 44
2B2 TBST 8.0 7.88 4.71 60
2B2 TBST 9.0 7.41 8.36 113
19F7 PBST 7.4 2.58 9.85 382
19F7 TBST 7.4 2.74 9.87 360
19F7 TBST 8.0 1.68 15.8 938
19F7 TBST 9.0 1.12 66.5 5924

a the standard error for the kinetic parameters in each global fit was ≤ 1%