Table 2. Kinetic analysis of purified rhPGRN binding to amine-coupled mAbs, as determined via SPR.
mAb | buffer | ka (1/Ms)a x 106 | kd (1/s)a x 10-4 | KD (pM) |
---|---|---|---|---|
2B2 | PBST 7.4 | 6.13 | 3.35 | 55 |
2B2 | TBST 7.4 | 7.69 | 3.35 | 44 |
2B2 | TBST 8.0 | 7.88 | 4.71 | 60 |
2B2 | TBST 9.0 | 7.41 | 8.36 | 113 |
19F7 | PBST 7.4 | 2.58 | 9.85 | 382 |
19F7 | TBST 7.4 | 2.74 | 9.87 | 360 |
19F7 | TBST 8.0 | 1.68 | 15.8 | 938 |
19F7 | TBST 9.0 | 1.12 | 66.5 | 5924 |
a the standard error for the kinetic parameters in each global fit was ≤ 1%