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. 2013 Nov 6;8(11):e80411. doi: 10.1371/journal.pone.0080411

Figure 1. DNA damage-inducing drugs cause premature senescence.

Figure 1

(A) HepG2 cells were cultured in RPMI medium with 0.1% DMSO or 10 µM etoposide for 0, 12, 24, 36 and 48 hours. (B) U2OS cells were cultured in RPMI medium with 0.1% DMSO, 2 µM etoposide, or 2 µM bleomycin for 0, 3, 5 and 7 days. For the assay of SA-β-Gal activity, cells stained with blue color were counted as described in Materials and Methods. The data (mean ± S.D.) were obtained from at least three independent experiments. Significant test results (P values) are shown.