Figure 4. Complementation of mutant 46B2 with bcal1124, but not bcal1124 with point mutations at R204H and E336G, results in restoration of plant killing.

Infection experiments were performed as described above following overnight growth of all strains (K56-2/pSCRhaTc, 46B2/pSCRhaTc, 46B2/p1124, 46B2/p1124”) in ½ LB + Tc100 + 0.02% w/v rhamnose. Infections omitted antibiotics but included 0.02% rhamnose to continue induction of the pSCRha promoter.