Table 1. Bacterial strains and species used in this study, including LD50 values.
Species | Strain | Source or relevant genotype or phenotypea | LD50 in L. minor (cfu/ml) +/- SEb | LD50 in G. mellonella (cfu) |
---|---|---|---|---|
Burkholderia cepacia | LMG 18821 | CF, Australia | 1.9x102 +/- 1.2x102 | 3.0x101 [13] |
LMG 2161 | Soil, Trinidad | 2.5x10° +/- 5.3x10-1 | 1.0x100 [13] | |
B. multivorans | C5393 | CF, Canada | >1.0x109 | >3.0x106 [13] |
C3430 | CF, Canada | >1.0x109 | >3.0x106 [13] | |
C5274 | CF, Canada | 3.1x109 +/- 2.4x109 | 1.0x106 [13] | |
C5568 | CF, Canada | 1.1x108 +/- 8.8x106 | >3.0x106 [13] | |
B. cenocepacia | PC715j | CF, Canada | 5.0x10° +/- 5.0x10-1 | 4.0x103 [13] |
J2315 | CF-e, UK | 1.6x106 +/- 7x105 | 1.0x105 [13] | |
K56-2 | CF-e, Canada | 1.2x101 +/- 7.0x10° | 9.0x102 [13] | |
C1257 | CF, Canada | 8.8x10° +/- 5.7x10° | 4.0x104 [13] | |
C4455 | CF, Canada | 1.01x105 +/- 3.9x104 | 1.0x105 [13] | |
C5424 | CF, Canada | 5.3x103 +/- 4.5x103 | 2.0x105 [13] | |
C6433 | CF, Canada | 2.8x104 +/- 2.2x104 | 3.0x104 [13] | |
Cep511 | CF, Australia | 7.6x10° +/- 3.4x10° | 8.0x108 [13] | |
K56-2 shvR::TpR | Plasposon insertion into bcas0225 | >3.0x108 | 100% virulence (i.e., ~9.0x102) [30] | |
B. stabilis | LMG 14294 | CF, Belgium | >1.0x109 | 2.0x106 [13] |
LMG 18870 | CF, Canada | >1.0x109 | >2.0x106 [13] | |
B. vietnamiensis | DBO1 | Soil, United States | 3.7x102 +/- 2.5x102 | 2.0x105 [13] |
PC259 | CF, United States | >1.0x109 | >3.0x106 [13] | |
B. dolosa | AU0645 | CF, United States | >1.0x1010 | >4.0x106 [13] |
STM1441 | Rhizosphere, Senegal | 4.8x107 +/- 1.3x107 | 4.0x104 [13] | |
B. ambifaria | Cep0996 | CF, Australia | 5.8x108 +/- 4.4x108 | 8.0x105 [13] |
AMMD | Rhizosphere, United States | 2.5x10° +/- 4.0x10-1 | n.d. | |
B. anthina | J2552 | Rhizosphere, UK | 4.5x108 +/- 5.2x107 | 3.0x105 [13] |
B. pyrrocinia | ATCC 15958 | Soil, Japan | 2.5x10° +/- 7.9x10-1 | 3.0x102 [13] |
Acinetobacter baumanii | AYE | Clinical, France | >1.0x109 | 2.01x105 [52] |
ACICU | Clinical, Italy | >1.0x109 | 5.58x105 [52] | |
ATCC 17978 | Clinical, France | >1.0x109 | 2.42x105 [52] | |
SDF | Human body louse | >1.0x109 | 4.84x107 [52] | |
Escherichia coli | E2348/69 | Parent strain | 9.0x101 +/- 3.8x101 | 2.57x103 [37] |
JPN15 | E2348/69 cured of EAF plasmid | 7.9x104 +/- 3.0x104 | 1.2x108 [37] | |
ΔescN | E2348/69 deficient in type III secretion | 6.9x102 +/- 4.7x102 | 1.82x105 [37] | |
ΔbfpA | E2348/69 deficient in bundle forming pilus | 6.0x102 +/- 5.9x102 | 4.9x103 [37] | |
ΔcpxR | E2348/69 deficient in Cpx pathway activation | 1.0x102 +/- 9.7x101 | 4.17x104 [37] | |
cpxA24* | E2348/69 with constitutive ON Cpx pathway | 6.3x105 +/- 4.9x105 | 2.5x1010 [37] | |
perA::KmR | E2348/69 deficient in type III secretion and bundle forming pilus | <1.0x103 | n.d. | |
Campylobacter jejuni | 11168 | Feces of diarrheic patient | >1.0x109 | n.d. [47] |
81-176 | Feces of diarrheic patient | >1.0x109 | n.d. [47] | |
Ralstonia solanacearum | GMI1000 | Tomato plant | >1.0x109 | n.d. |
Abbreviations: CF, cystic fibrosis infection; CF-e, strain that has spread epidemically among CF patient; n.d., not determined.
S.E., standard error of the mean, included where applicable