Table 2. Attenuated B. cenocepacia K56-2 plasposon mutants identified against individual duckweed plants.
| Strain | LD50 cfu/mL +/- SEa | Plasposon insertion locusb | Characteristics | Prevalence among sequenced Burkholderia species |
|---|---|---|---|---|
| WT | 1.2x101 +/- 7.0x10° | n/a | n/a | |
| 11G10 | >1x109 | bcas0210: Antifungal compound synthase (AFC) cluster | AFC cluster genes influence biofilm formation, lipid metabolism, swarming motility and antifungal activity [40] | B. cenocepacia, B. ambifaria, B. lata, B. pseudomallei, B. mallei |
| 12C9 | 2.1x105 +/- 4.6x104 | bcal0311: HisG (ATP phosphoribosyltransferase) | Part of histidine biosynthesis cluster; upregulated 2-fold during chronic CF infection [46] | Ubiquitous |
| 16A11 | ~1x106 | bcal2159 | Hypothetical α/β barrel domain-containing protein; downstream from SuhBBc [42] | B. cenocepacia, B. lata, B. multivorans, B. gladioli, B. xenovorans |
| 32B11 | 1.9x103 +/- 1.5x103 | Intergenic between bcal0549 and bcal0550 | n/a | |
| 42H4 | 3.5x104 +/- 1.7x104 | n.d. | ||
| 46B2 | 7.7x101 +/- 1.8x101 | bcal1124: Hypothetical protein in BcenGI5 genomic island | Putative viral origin of replication-binding protein | B. cenocepacia, B. pseudomallei |
| 50D9 | >2.6x108 | bcas0225: ShvR | LysR-like protein regulates AFC cluster, quorum sensing, protein secretion, protease and lipase production, and virulence [8,39,46] | B. cenocepacia, B. ambifaria, B. lata, B. gladioli |
| 51H6 | ~1x103 | bcas0134: LysR-like regulator | Putative LysR-like regulator, regulated by BDSF signalling [41] | Limited to B. cenocepacia ET-12 epidemic lineage |
| 62F12 | ~5x105 | bcal0870 | Putative oxidoreductase, identified as an essential gene in B. cenocepacia H111 [43] | Ubiquitous |
a
S.E., standard error of the mean, included where applicable
b
n.d., not determined.