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. 2013 Nov 6;8(11):e79950. doi: 10.1371/journal.pone.0079950

Figure 3. A) SNP-genotyping showing significant loss of heterozygosity throughout the genome.

Figure 3

B) A heatmap showing relative expression of the 100 genes most strongly overexpressed in the xenograft compared with classic chordomas. Chromosome location of each gene is denoted along the right side. Green represents relative underexpression, and red represents relative overexpression. The three columns on the left are data from the comparison chordomas, and the two columns on the right are from the xenograft. C) RT-PCR confirmation of gene expression in the xenograft. cDNA reverse transcribed from RNA isolated from the xenograft and from the JHC7 cell line was amplified with primers for the indicated genes. Lanes 1 are reactions using RNA from JHC7, and Lanes 2 are reactions using RNA from the xenograft. Ribosomal RNA 36B4 was used as a positive control to confirm cDNA integrity (data not shown).