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. 2013 Nov 6;8(11):e79961. doi: 10.1371/journal.pone.0079961

Figure 2. Bacterial burdens of extraintestinal organs upon K. pneumoniae infection in diabetic and naïve mice.

Figure 2

Liver (A and E), spleen (B and F), kidneys (C and G), and blood (D and H) were retrieved at 72 hpi from the naive mice (NI) and diabetic mice (DI) which were orally inoculated with 3 × 108 CFU of log-phased K. pneumoniae CG43-luxCDABE. Ten-folded dilutes of the tissue homogenates were plated onto LB-TcR agar to enumerate CFU. Bacterial burdens were represented as Log CFU/g for tissues and Log CFU/ml for blood. Data from all the K. pneumoniae-inoculated naïve and diabetic mice were shown in A to D. Horizontal bars indicate geometric means. The limit of detection was approximately 10 CFU. Samples which yielded no colonies were plotted having the value as 10 CFU g-1 tissues. For emphasizing the extraintestinal dissemination of K. pneumoniae, samples that yielded no colonies in extraintestinal tissues were considered non-infected. Only data from the K. pneumoniae-positive samples were plotted in E to H. Statistical analysis by the Mann-Whitney U test (one-tailed) showed no significant difference between NI and DI groups. The sample sizes in A-D are 13 and 10 in NI and DI groups and are 4 and 8 in E-H for NI and DI groups, respectively.