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A - C, BGN immunostaining was performed on paraffin-embedded tissue sections. (A), Ta; (B), T2; (C), T4. (D), quantitative image analysis of BGN in non-invasive tumors (Ta, T1) and muscle-invasive tumor stages (T2-T4). (E), correlation of BGN positive area fraction with tumor staging. n=5 patient biopsies of each tumor stage, ***p<0.0001, **p=0.0025.
