Figure 1. Adhered/internalized viable and heat killed C. jejuni per A. polyphaga trophozoite.

After 1-incubation the majority of amoeba associated viable C. jejuni were found on the surface of trophozoites but at 24 h and thereafter, the majority were found in intracellular vacuoles. The number of viable bacteria internalized into A. polyphaga trophozoites was significantly higher than for heat killed bacteria during the first 48 h. Data are based on three to five independent experiments for samples taken at 1 h–72 h and on one experiment for 96 h. Mean±95 confidence interval.