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Small amounts of highly diluted P66 (1∶1,000 in 1% Genapol) was added to both sides of a diphytanoyl PC membrane. After reconstitution of one single 11 nS P66 unit, 90 mM PEG 400 was added to both sides of the membrane. The P66 conductance was blocked stepwise exhibiting subconductance steps of approximately 1.5 nS; temperature = 20°C; applied voltage = 20 mV.
