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. 2013 Nov 6;8(11):e78410. doi: 10.1371/journal.pone.0078410

Table 1. Concordance values for samples passing both quality check values.

Samples passing QC Whole exome Whole genome
Nr. of alleles typed Concordance% Nr. of alleles typed Concordance%
HLA-A 340 92.3 52 78.9
HLA-B 360 96.7 60 85.0
HLA-C 322 92.6 24 95.8
All the genes 1022 93.9 136 84.6

Quality check (QC) was applied in these subsequent steps: i) only those samples were processed where the length of the read was longer than 76 bps for both ends ii) after alignment the percentage of the covered region (Inline graphic) was calculated for exons 2 and 3. If any of these exons were covered by reads only less than 70% of the extent of the exon, the sample failed QC and was discarded. iii) finally, using this formerly calculated Inline graphic value, if the average Inline graphic for exons 2 and 3 was less than 80%, the sample was discarded. Average coverage depth was not considered since Inline graphic was a better indicator of correct concordance.