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Optical images of zebrafish embryos used to incubate with the Ag NPs for 2 h: (A) cleavage (2 hpf), (B) early-gastrula (6 hpf), (C) early-segmentation (12 hpf), (D) late-segmentation (21 hpf), and (E) hatching stage (48 hpf) embryos. Here we name them (A–E) as stage I–V embryos, respectively. Scale bar is 500 μm.
