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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: Chem Res Toxicol. 2013 Sep 11;26(10):10.1021/tx400228p. doi: 10.1021/tx400228p

Figure 4.

Figure 4

Study of dose and stage-dependent effects of the Ag NPs on embryonic developments. Histograms show that the percentages of stages I–V embryos that have been incubated with (A–E) the Ag NPs or (F) supernatant for 2 h, develop to normal and deformed zebrafish or die in egg water over 120 hpf. The 48 embryos at each given stage are used for 4 repeated experiments. Each experiment includes 4 replicated measurements with 4 embryos per measurement. The means and standard deviations (error bars) of each four replicated experiments are presented. The supernatant concentration (24 pM) represents the same dilution factor of the supernatant collected from the last-washing of NPs as those prepared for 24 pM NPs from the original NP solution.