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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: J Med Chem. 2013 Jun 25;56(13):10.1021/jm400575x. doi: 10.1021/jm400575x

Figure 4.

Figure 4

6t inhibit in vitro tubulin polymerization. A. Purified tubulin was incubated in the presence of 10 μM concentration of taxol, nocodazole, 6t and DMSO at 37 °C. The extent of tubulin polymerization was determined by the increasing absorbance at 340 nM over 40 min. 6t and nocodazole (a known tubulin depolymerizer) were able to completely inhibit tubulin polymerization compared to Taxol (tubulin polymerizer) and DMSO suggesting that 6t interfere with normal tubulin polymerization kinetics. Data shown are representative of three independent experiments. B. Purified tubulin was incubated in the presence of 10 μM concentration of 6t, 25a, 25c and DMSO at 37 °C. The extent of tubulin polymerization was determined by the increasing absorbance at 340 nM over 40 min. All three compounds were able to completely inhibit tubulin polymerization suggesting that these compounds interfere with normal tubulin polymerization kinetics. Data shown are representative of three independent experiments.