. Author manuscript; available in PMC: 2014 Jul 11.

Published in final edited form as: J Med Chem. 2013 Jun 25;56(13):10.1021/jm400575x. doi: 10.1021/jm400575x

Table 3.

In vitro cytotoxicity profiles of most active molecules 6i, 6p, 6t and 25c in various tumor cell lines.

Cell Line	Tumor Type	IC₅₀ (>M)^a
Cell Line	Tumor Type	6i	6p	6t	25c
DU145	Prostate	0.075	0.050	0.008	0.15
K562	Leukemia	0.075	0.015	0.003	0.01
LNCAP	Prostate	0.100	0.050	0.008	0.10
PC3	Prostate	0.100	0.025	0.008	N/D
SK-MEL-28	Melanoma	0.050	0.025	0.008	N/D
MCF-7	Breast	0.050	0.025	0.004	0.08
SK BR-3	Breast	0.050	0.025	0.004	N/D
BT474	Breast	0.250	0.025	0.010	0.15
BT20	Breast	0.250	0.250	0.008	0.10
T47D	Breast	0.100	0.025	0.008	N/D
A431	Epidermoid	0.030	0.600	0.005	0.15
HCT116	Colo-Rectal	0.100	0.010	0.004	N/D
HCT15	Colo-Rectal	0.100	0.025	0.008	0.20
SKOV3	Ovarian	0.100	0.025	0.008	N/D
OVCAR3	Ovarian	0.100	0.100	0.004	N/D
H187	NSCLC	0.100	0.010	0.004	N/D
N417	SCLC	0.050	0.010	0.004	0.03
RF-48	Gastric	0.025	0.010	0.004	N/D
RF-1	Gastric	0.050	0.010	0.004	N/D
MIAPaCa2	Pancreatic	0.100	0.010	0.004	0.08
H80	Glioma	0.100	0.025	0.004	N/D
MES-SA	Uterine	0.050	0.025	0.004	N/D
NAMALWA	Lymphocytic	0.100	0.025	0.008	N/D
DAUDI	Lymphocytic	0.100	0.025	0.008	0.03
MOLT-4	Lymphocytic	0.100	0.025	0.008	0.03
U87	Glioma^b	0.229	0.026	0.009	0.09

GI₅₀ (growth inhibitory concentration-50%). Cells were treated with 6i, 6p, 6t and 25c in a 96 h dose response assay. The GI₅₀ concentration (μM) was determined from duplicate cell counts using a Hemocytometer following trypsinization and trypan blue staining.

Values are IC₅₀ as measured by SRB assay. ND = not determined