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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Aging Cell. 2013 Jul 29;12(6):10.1111/acel.12127. doi: 10.1111/acel.12127

Fig. 3. Lipoic acid restored the age-induced imbalance of Akt/JNK signaling.

Fig. 3

Equal amount of homogenate samples from brain cortices of Fischer 344 rats were loaded on the gel. (A) Decreased activation (phosphorylation at Ser473) of Akt with age. (B) Decreased inactivation (phosphorylation at Ser9) of GSK3β with age. (C) Increased activation of JNK with age; lipoic acid increased JNK phosphorylation in the brain cortex homogenate from 6 month-old rats but decreased its phosphorylation in the brain cortex homogenate from 24 month-old rats. (D) Lipoic acid restored the age-associated imbalance of Akt/JNK signaling. Relative activity of JNK and Akt signaling was determined by assessing the relative active JNK and Akt levels on the same membrane. (E) Increased IRS1 Ser307 phosphorylation with age and reduction by lipoic acid. (F) Decreased IRS1 Tyr608 phosphorylation with age and restoration by lipoic acid.*p < 0.05, **p < 0.01, n ≥ 6.