Fig. 5.

Immunolocalization of BBS5 in Xenopus distal retina. a Cryosections of dark-adapted Xenopus retina were stained with anti-BBS5 monoclonal (green) and anti-Arr1 rabbit polyclonal antibody (a′′; red). The merge image (a′) indicates co-localization of BBS5 and Arr1 along the axoneme and inner segments (IS) of the rod photoreceptors (white arrowheads mark two axonemes that are stained for both Arr1 and BBS5). b Additional cells that also stained for BBS5 (white arrow in both a, b), but not for Arr1, were identified as cones by staining for arrestin4 using a monoclonal anti-cone arrestin4 with an isotype-specific secondary (green). c In cryosections of light-adapted Xenopus retina, BBS5 (green) remains along the axonemes and inner segments, whereas Arr1 (red) principally localizes to the rod outer segments (OS). d A control section stained using BBS5 monoclonal (green) pre-absorbed to heterologously expressed BBS5 protein demonstrates specificity of the monoclonal antibody. Scale bar 20 μm