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TTD values for (A) Staphylococcus aureus, (B) Escherichia coli, and (C) Pseudomonas aeruginosa preincubated in aerobic and anaerobic blood culture bottles at 25℃ or 37℃ prior to insertion into the BacT/Alert 3D system. False negatives were detected after 48 hr of prestorage at 25℃ and after 24 hr of preincubation at 37℃ for P. aeruginosa. Bacterial input concentration was 15 colony-forming units/mL.
Abbreviation: TTD, time to detection.
