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. 2014 Jan;4(1):47–51. doi: 10.1016/S2221-1691(14)60207-0

Table 1. List of primers used for amplification of COI and COII genes from mtDNA of the An. stephensi and detection of malaria sporozoites in mosquitoes.

Primer name sequence 5′-3′ Expected amplicon length (bp)
COI Forward 5_-TTG ATT TTT TGGTCA TCC AGA AGT-3 877
Reverse 5-TAG AGC TTA AAT TCA TTG CAC TAA TC-3
COII Forward 5_- ATG GCA ACA TGA GCA AATT-3 640
Reverse 5_-CCA CCC TTT CTG AAC ATT GAC C-3
rPLU5 Forward 5′ CCTGTTGTTGCCTTAAACTTC 3′ 1 200
rPLU6 Reverse 5′ TTAAAATTGTTGCAGTTAAAACG 3′
rFAL1 Forward 5′ TTAAACTGGTTTGGGAAAACCAAATATATT 3′ 205
rFAL2 Reverse 5′ ACACAATGAACTCAATCATGACTACCCGTC 3′
rVIV1 Forward 5′ CGCTTCTAGCTTA ATCCACATAACTGATAC 3′ 120
rVIV2 Reverse 5′ CTTCCAAGCCGAAGCAAAGAAAGTCCTTA 3′