Table 1. List of primers used for amplification of COI and COII genes from mtDNA of the An. stephensi and detection of malaria sporozoites in mosquitoes.
Primer name | sequence 5′-3′ | Expected amplicon length (bp) |
COI | Forward 5_-TTG ATT TTT TGGTCA TCC AGA AGT-3 | 877 |
Reverse 5-TAG AGC TTA AAT TCA TTG CAC TAA TC-3 | ||
COII | Forward 5_- ATG GCA ACA TGA GCA AATT-3 | 640 |
Reverse 5_-CCA CCC TTT CTG AAC ATT GAC C-3 | ||
rPLU5 | Forward 5′ CCTGTTGTTGCCTTAAACTTC 3′ | 1 200 |
rPLU6 | Reverse 5′ TTAAAATTGTTGCAGTTAAAACG 3′ | |
rFAL1 | Forward 5′ TTAAACTGGTTTGGGAAAACCAAATATATT 3′ | 205 |
rFAL2 | Reverse 5′ ACACAATGAACTCAATCATGACTACCCGTC 3′ | |
rVIV1 | Forward 5′ CGCTTCTAGCTTA ATCCACATAACTGATAC 3′ | 120 |
rVIV2 | Reverse 5′ CTTCCAAGCCGAAGCAAAGAAAGTCCTTA 3′ |