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Investigation of the transport of 3H-GSH into wtABCG2-containing insect vesicles. Uptake of 500 μM 3H-GSH was measured in Sf9 vesicles expressing ABCC1, wtABCG2, or inactive ABCG2, for 5 min at 37°C. ATP-dependent transport with or without the ABCC1- or ABCG2-specific inhibitor, 4 μM MK-571 or 1 μM Ko143 respectively, is shown. Bars represent the average of three independent measurements ± SD values. t-test analysis: ***p < 0.001.
