Skip to main content
. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Clin Cancer Res. 2013 Jun 26;19(16):10.1158/1078-0432.CCR-12-2169. doi: 10.1158/1078-0432.CCR-12-2169

Figure 2.

Figure 2

Lack of anergy induction by β-ManCer cannot be explained by selective Vβ usage of activated iNKT cells. Mice were injected with 2.4 nmol (2µg) β-ManCer or vehicle control. Splenocytes were harvested at 4 weeks, labeled with CFSE, and restimulated in vitro with 500 nM β-ManCer or vehicle for 3.5 days. At the conclusion of the restimulation, cells were harvested and analyzed by flow cytometry for Vβ expression on iNKT cells. Samples were gated on iNKT cells (defined as CD3intermediateα-GalCer/CD1d tetramer+) expressing TCRβ, Vβ2, Vβ7, and Vβ8.1/8.2. The percentage of Vβ+ iNKT cells that had diluted CFSE was determined by analysis in FlowJo. Bars represent the percentage of Vβ+ iNKT cells in each group that diluted CFSE. Spleens from at least 3 mice were pooled for each group. A representative of 2 reproducible experiments is shown.