Figure 5.
iNKT cells primed by β-ManCer, but not α-GalCer, can respond to restimulation to induce tumor protection. Mice were injected with 2.4 nmol (2 µg) α-GalCer or β-ManCer or vehicle control. Two months later, CT26 cells (5 × 105) were injected i.v. into the tail vein of BALB/c wild type and 50 pmol of glycolipids were administered within one hour after tumor challenge. Mice were sacrificed 14 days after tumor challenge and lung metastases were enumerated. Tumor burden was calculated by dividing the number of lung tumors in the treated groups by the mean number of tumor nodules in the respective vehicle control groups. Representatives from at least 2 independent experiments are shown. Symbols represent the tumor burden of individual mice, lines and error bars represent mean±SD. *, statistically significant vs vehicle control, p<0.05