Figure 3.
PFTS protects against acute pancreatitis-accelerated PanIN formation. (A) Cerulein treatment protocol. Mice with activated Kras (p48-Cre;LSL-KrasG12D) were injected with cerulein and killed 9 days post-injection. At the day of the first injection of cerulein, mice were treated with PFTS (100 mg/kg per day) as shown. (B) The following stains were performed. (Top) hematoxylin and eosin (H&E) stain. Fewer ductal and more acinar structures were observed in the PFTS-treated mice (x4); scale bars, 200 µm. (Middle) Amylase. PFTS-treated mice retained larger areas of amylase-expressing epithelium (x10); scale bars, 100 µm. (Bottom) Alcian blue stain (x20); scale bars, 50 µm. (C) The percentages of amylase-positive area and Alcian blue-positive staining per field were quantified and expressed as means ± SEM (*P < .05 for both).